| Since 1966 bacteriologists have come to respect the
May impingers for the separation of particles according
to their deposition sites in the respiratory system. The
fractions are collected gently into liquid where clumps
separate into viable units. There is little danger of
sample overload and sub-samples permit the use of a
variety of culture methods. The original designs
involved complex glass blowing, were difficult to clean
and to reproduce accurately. The 20 l/min size is
designed to produce particle fractions (> 10 µm, 10-4
µm, < 4 µm) that are closest to those likely to be
agreed as international standards.
The Burkard version closely follows May's intermediate
(20 l/min) size, accurately produced in anodised
aluminium alloy (or stainless steel). Airtightness is
ensured by vertical compression, releasing this allows
the sampler to be dismantled for removal of the catch,
and for cleaning. It can then be fitted and re-assembled
before heat sterilisation and operation. The flow is
controlled by a critical orifice built into the third
stage. To ensure this accurate flow the vacuum pump must
be capable of maintaining a pressure difference of at
least 180 mm (7inches) of mercury. A 'stagnation-point
shield' is provided to improve aspiration efficiency in
moving air. To maintain the designed depth of collection
fluid in the machined chambers it is recommended that 6
ml be added to each stage. (Not 4 ml as originally
described).
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